Characteristics of Chromosomes and Electrophoretic Patterns of Muscle Protein and Lactate Dehydrogenase in Fish of Genus Carassius (Cypriniformes)

Abstract

Chromosomes and electrophoretic patterns of muscle protein and of LDH isozymes in the Japanese funa Carassius auratus were studied comparatively. All the specimens examined were either of diploid or triploid types. Electrophoretic patterns of muscle protein (MP patterns) were divided into three basic types. The specimens with Type I were the diploid type excepting the gengorobuna with Type III, and those with Type II were the triploid type. LDH isozyme patterns were divided into three types. Type L-1 was found in the kinbuna, and Type L-2 was in all the specimens excepting the gengorobuna with Type L-3 and the kinbuna. All the diploid specimens were the same karyotype constitution and the specimens with the exception of the kinbuna had no C-banded marker chromosomes. The triploid specimens with the different MP patterns showed different karyotypes and C-banding patterns, suggesting the different origins. As a result, the Japanese funa may be classified into four groups, the kinbuna, the diploid ginbuna, the triploid ginbuna, and the gengorobuna. This classification seems to represent well the condition of speciation and evolution.