Isolation of Single Viable Polytene Cells from Larval Oenocytes of Acricotopus lucidus (Diptera, Chironomidae)

抄録

Oenocytes explanted and collected from prepupae of Acricotopus lucidus (Diptera, Chironomidae) were enzymatically dissociated into single cells by treatment with elastase. The isolated cells were kept viable for days over HeLa cells as feeder culture. Viability of the isolated cells was tested with flourescein diacetate and Trypan Blue. Carmine-orcein staining demonstrated that the oenocyte nuclei contain polytene chromosomes with distinct banding patterns. The DNA-content and polytenic level of prepupal oenocyte nuclei were measured using flow cytometry at 128 C. The morphology of oenocytes was investigated in differential interference contrast and with transmission electron microscopy.