Mutagenic Potential of the Human Intestinal Amoeba, Entamoeba histolytica Assayed in Mice as Model
1991 年 56 巻 4 号 p. 613-619
詳細
1991 年 56 巻 4 号 p. 613-619
The mutagenic potential of the human intestinal amoeba, Entamoeba histolytica, NIH: 200 strain in TYI-S-33 culture medium added with sterile bovine serum, vitamins and antibiotics was intraperitoneally injected to individual mouse, Mus muculus a dose of 2ml containing 15×105 amoebae after 72 hr incubation and parallely another set of mice weighing about 20g were intraperitoneally injected to serve as control, was assessed at 30 min, 24 hr, 48 hr and on 7 th day after injection. The net increase over control in the E. histolytica treated mice in the combined data of all intervals was 9.94% for bone marrow chromosome aberration, 0.33% for micronucleated erythrocytes, 15.0% for first spermatocyte metaphase chromosome aberrations and 1.55 % for sperm with abnormal head frequency. The difference was statistically significant in each test.
