Chromosomes and Nuclear DNA in Regenerants of Scilla indica (Roxb.) Baker Derived from Two Explant Sources

抄録

Chromosome analysis of regenerants of Scilla indica (Roxb.) Baker obtained through organogenesis from leaf callus and somatic embryogenesis from anther callus was carried out. The plants regenerating from the leaf callus were all diploids whereas those regenerating through somatic embryos from anther cellus showed both diploid and mixoploid plantlets. The karyotype of the regenerants did reveal major structural changes in the diploid plantlets derived from both callus lines. In situ nuclear DNA estimation of the regenerants indicated incerase in mean DNA value only in case of a few mixoploid plantlets regenerated from anther callus via somatic embryogenesis. The nuclear state of the explant thus has an important role to play in determining the cytological status of regenerants.