Chromosomal Localization of 5S rRNA Genes in Vicia faba and Crepis capillaris

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Chromosomal location of 5S rRNA genes in Vicia faba and Crepis capillaris was investigated by in situ hybridization using probes amplified by the polymerase chain reaction (PCR) using a set of universal primers of parts of 5S rRNA sequence and labeled with biotin. 5S rRNA genes were located at two sites on the satellite of the large metacentric chromosomes in V. faba and were at the short arm of telocentric chromosomes which was very close to the site of large rRNA genes in C. capillaris.