Colchicine-lnduced Autotetraploidy in Clitoria ternatea L

抄録

Seeds and seedling of Clitoria ternatea L. white (Family Papilionaceae) were treated with colchicine solution from 0.05% to 0.25% concentrations for different durations. Shoot immersion method resulted in 4 colchiploid (C₀) plants from which 10 C₁ plants were obtained. Expression of gigantism in morphological characters such as plant height, size of leaflets, flowers, seeds and pollen diameters was noted in these autotetraploids with significant decrease in stomatal frequency, percent pollen fertility and seeds/pod ratio. Karyotypic study of the autotetraploid plant indicated 2n = 32 with all submedian chromosomes.
Chromosome association at diakinesis and metaphase-I revealed gradual decrease in quadrivalent frequency with increase in bivalent frequrncy from C₀ to C₁ generation. Meiotic abnormalities were also reduced in C₁ plants as compared to C₀ plant.