Estimation of the Nuclear DNA Content of Strawberries (Fragaria spp.) Compared with Arabidopsis thaliana by Using Dual-step Flow Cytometry

抄録

Genus Fragaria consists of several species, ranging from diploid to octoploid, and including various types of sex determination. The genome size of Fragaria species is not known correctly, mainly because preparation of samples suitable for genome size estimation has been difficult. Moreover, it is difficult to choose a suitable standard material for estimation given the small genome size of Fragaria species. Our experimental data showed that the flower petal is a more suitable tissue for flow cytometry. We developed a new procedure, which prevents contamination of tissue samples with debris, and thus allows more accurate estimation of the DNA content during flow cytometry. Arabidopsis thaliana has been sequenced nearly complete, and the estimated genome size from the sequence data is 125 Mbp. We chose Arabidopsis thaliana as standard material. Using this approach, we successfully estimated the genome size of the diploid Fragaria species to be around 164 Mbp/C.