The Chromonemata Observed in the Fresh Pollen Mother-cells of Trillium kamtschaticum Pall. Mounted wich Saccharose Solution

Abstract

1) Fresh pollen mother-cells at prophase I-anaphase I of Trillium kamtschaticum were observed in anthral slime mixed with an ample amount of 6.0 or 7.0g./100 c.c. saccharose solution.
2) Cell division was inhibited after preparation, but the chromosomes were always distinctly visible, except in the earlier prophase I cells.
3) The internal structure of the chromosomes was examined in fresh state and the presence of double coiling in chromonemata at late prophase I-late anaphase 1 was demonstrated.
4) Indications for the existence of double-coiled twin chromonemata in a “single major spiral” or a “monad” at metaphase I and anaphase I were presented in some detail.
The writer wishes here to express his sincere gratitude to Prof. T. INUKAI, Prof. T. SAKAMURA, and Prof. H. MATSUURA for their encouragement and criticisms during the course of the present work. Acknowledgement is also due to the Foundation for the Promotion of Scientific and Industrial Research of Japan, with the aid of whose financial support the present investigation has been carried out.