2002 Volume 12 Issue 1 Pages 33-37
To date, the appropriate condition for the collection and storage of samples in flowcytometorical analysis has not been studied. In this study, flowcytometorical analysis was performed using different tubes (EDTA,heparin, RPMI with heparin) at different points (within 6 hours or at 24hours) from sample collection, and the influence by changes of storage conditions on the results of flowcytometorical analysis was examined. When the analysis was performed within 6hours, more than 85% of cells were viable, and there was no change in fluorescence intensity or positive rate in all tubes. However, the percentage of dead cells was increased in the samples kept in the EDTA tube for 24 hours. Furthermore, a couple of samples showed the changes of fluorescence intensity and scatter of the cytogram after 24 hours regardless of the kinds of tubes. These results suggest that a careful review of the results is necessary for an appropriate report of flowcytometorical analysis, especially when samples left for long time after collection are applied.
