Problems of Flow cytometric gating for hematopoietic cell analysis in clinical test.

Abstract

Flow cytometric analysis is indespensable for identifying immunophenotypically hematopoietic cells. In such analysis, it is the most important issue how to choose flow-cytometrically target cells, namely gating. To differentiate target cells from the other cells, three gating approaches are generally known; morphological gating derived from the SSC/FSC cytogram, specific gating of the Leuko-gate(BD) using CD45 and CD14 measurement, and two-color gating using two CD antibodies relevant to target cells. In this paper, we reviewed our experiences of the problematic issues and relevance of gating in flow cytometric analysis as a routine hematological test.