FACS analysis of the cord blood hematopoietc stem cell during ex vivo expansion

Abstract

Ex vivo expansion of CD34 positive cells in the umbilical cord blood (UCB) was investigated in liquid culture system with various combination of cytokines (stem cell factor [SCF], Thrombopoietin [TPO], interleukin [IL]-6,soluble IL-6 receptor, Flt-3/Flk-2 Ligand) and FACS analysis of cell surface markers indicated an absolute CD34+ cell numbers increased after 12 days of culture ( 20 -fold) . And these cytokines were significantly effective in increasing non-lineage-committed hematopoietic progenitor cells (CD34+CD38- subpopulation) ,though CD34+CD133+ subpopulations were slightly decreased. The tendency to decrease of CD90 and CD117 after 12days culture were also observed though a big change was not seen for CD123 and HLA-DR after 12days culture.