2005 Volume 15 Issue 1 Pages 27-33
The polyploidization of tetraploid Meth-A cells by staurosporine (SS), an inhibitor of protein kinases, was examined. Tetraploid Meth-A cells were polyploidized well by SS, but diploid cells were not. When the drug concentration was gradually reduced, polyploidization progressed further. Cell clones having octaploid and decaploid DNA content were obtained from the cell population 20 days after the drug removal. The durations of the G1, S and G2/M phases of the polyploid cells were almost the same as those of the parent tetraploid cells. These findings suggest that polyploidization by SS is responsible for the production of polyploid Meth-A cells whose ploidy is not a power of 2.
