Ex Vivo増幅培養にともなう臍帯血造血幹細胞のFACS解析

抄録

Ex vivo expansion of CD34 positive cells in the umbilical cord blood (UCB) was investigated in liquid culture system with various combination of cytokines (stem cell factor [SCF], Thrombopoietin [TPO], interleukin [IL]-6,soluble IL-6 receptor, Flt-3/Flk-2 Ligand) and FACS analysis of cell surface markers indicated an absolute CD34+ cell numbers increased after 12 days of culture ( 20 -fold) . And these cytokines were significantly effective in increasing non-lineage-committed hematopoietic progenitor cells (CD34+CD38- subpopulation) ,though CD34+CD133+ subpopulations were slightly decreased. The tendency to decrease of CD90 and CD117 after 12days culture were also observed though a big change was not seen for CD123 and HLA-DR after 12days culture.