ホルマリン固定パラフィン包埋組織切片を用いたin situ RT-PCR法について

抄録

Because RNA is easily degraded by RNase that is present in tissues handled by routine methods of surgical pathology, it is sometimes difficult to obtain satisfactory results in in situ hybridization for mRNA on archival formalin-fixed, paraffin-embedded tissue sections. In situ reverse transcription-polymerase chain reaction (in situ RT-PCR) is a technique that allows in situ amplification of reverse-transcribed complementary DNA (cDNA) originating from mRNA. Because in situ RT-PCR amplifies cDNA on a tissue section, it is considered theoretically more suitable for in situ detection of target mRNA than conventional in situ hybridization. In the present article, we reviewed and discussed in situ RT-PCR with special reference to the technical aspects.