We have commercialized the world's first damage-less cell sorter (PERFLOW® Sort: http://www.furukawa.co. jp/bio/english/) for analyzing and sorting living cells, especially human induced pluripotential stem (iPS) cells and human embryonic stem (ES) cells. We have succeeded in generating monoclonal human iPS cells from single cells with high survival of 66%. Different from the conventional sorting methods using ultrasonic wave, charge and high pressure, which bring physical damage to cells, PERFLOW Sort adopts high-performed micro-flow control and fixed optical fiber alignment to monitor flow speed of target cells precisely, as guarantees living cells to be sorted with less physical damage. On the other hand, PERFLOW Sort adopts transmitted light signals instead of conventional forward scatter to achieve high-sensitivity analysis.
