Flow cytometry is a measuring device frequently used in basic research, in our facility, we have developed a fully automatic fl ow cytometer device that performs cell isolation, staining of cell nuclei, and measurement, and is using it for intraoperative diagnosis. Placing a small specimen in a reagent containing PI calculates, the total number of cells, the DNA histogram, and the Malignancy Index (MI), which means in the proliferation phase, the ratio of the number of cells (S, G2, M) / total number of cells. High MI reveals the appearance of DNA aneuploidy and an increase in cells in the proliferative phase. The major feature of this device is that calculating takes only within 10minutes by specializing in DNA amount analysis and thoroughly reviewing the process. Therefore, we can evaluate the malignancy of tumors in a short time. This report introduces our previous study of this device, stump diagnosis, WHO grading, prognosis in glioma, and differentiation from primary central nervous system lymphoma, then how to use it in decision making in the operation. Intraoperative flow cytometry has enabled various intraoperative decision-making support by converting rapid intraoperative diagnosis, which was an analog-like transmission, into digitized histological information. In the future, the development of this research makes expectations for more accurate diagnosis and prediction by artifi cial intelligence and the development of other departments.
