2010 Volume 25 Issue 6 Pages 565-572
It has been demonstrated that β-1,3-glucans can form novel complexes with homo ODNs via a combination of hydrogen bonding and hydrophobic interactions. Dectin-1 is a major receptor involved in the recognition of β-glucans on APCs, including macrophages, dendritic cells, monocytes, neutrophils, and a subset of T cells. Therefore, the complex could be used to deliver an antisense ODN that can rectify abnormalities associated with inflammation. In this study, we show the application of the novel complex consisting of β-1,3-glucan and antisense oligonucleotide (AS-ODN) or CpG-DNA for drug delivery system. In-vivo, CpG-DNA/SPG induced robust type interferon responses dependently of Toll-like receptor 9. AS-ODN/SPG reduced the inflammation of dextran sulfate-induced colitis.
