Abstract
In the cosmetics industry, where the development and utilization of alternatives to animal testing is being promoted, a method to develop individual tests and evaluate them in combination according to the Key Events of the Adverse Outcome Pathway has been proposed and utilized as an alternative method for local toxicity. On the other hand, for systemic toxicity, recently, the concept of Next Generation Risk Assessment has been proposed, and there have been many reports on case studies that combine New Approach Methodologies tests in accordance with scenarios tailored to the characteristics of compounds, with the ultimate goal of protecting human health. Although many issues remain for alternative methods to animal testing, we would like to introduce some cases here, as they are expected to be developed and utilized in a variety of fields in the future.
