Abstract
Selection of target sites is one of key issues in knock-down experiments using siRNA. So far, our systematic analysis of siRNA effects at various target sites revealed that siRNA activities are dependent on some nucleotides at specific position of target sequences and tight secondary structure of target messages, but are independent of the position in the target message. Here we review recent findings concerning mechanism of RNAi and factors that affect siRNA activities, and provide a basis for designing siRNA and siRNA expression vector.
