Abstract
Silencing of gene expression by siRNA is one of the powerful tool for genetic analysis in vitro and in vivo. siRNA technique is simple and its effect is sharp rather than antisense DNA, so many feasibility studies for its therapeutic use have been conducted. However, the utility of siRNA for therapeutic application is hampered by absence reliable and safe delivery method. Recently, we have established atelocollagen-mediated delivery system for therapeutic use of nucleic acid medicines such as plasmid DNA and antisense DNA. In this article, application of siRNA to our delivery system and its effectiveness in animals was described.
