Drug Discoveries & Therapeutics
Online ISSN : 1881-784X
Print ISSN : 1881-7831
ISSN-L : 1881-7831
Original Articles
Biophysical characterization of a model antibody drug conjugate
Tsutomu ArakawaYasunori KurosawaMichael StormsToshiaki MaruyamaC. J. OkumuraNasib Karl Maluf
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2016 Volume 10 Issue 4 Pages 211-217

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Abstract

Antibody drug conjugates (ADC) are important next-generation biopharmaceuticals and thus require stringent structure characterization as is the case for monoclonal antibodies. We have tested several biophysical techniques, i.e., circular dichroism, analytical ultracentrifugation, differential scanning calorimetry and fluorescence spectroscopy, to characterize a fluorescein-labeled monoclonal antibody as a model ADC. These techniques indicated possible small structure and stability changes by the conjugation, while largely retaining the tertiary structure of the antibody, consistent with unaltered biological activities. Thus, the above biophysical techniques are effective at detecting changes in the structural properties of ADC.

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© 2016 International Research and Cooperation Association for Bio & Socio-Sciences Advancement
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