Anti-virulence activities of biflavonoids from Mesua ferrea L. flower

Xiaochun Zhang; Rongrong Gao; Yan Liu; Yuhe Cong; Dongdong Zhang; Yu Zhang; Xuefei Yang; Chunhua Lu; Yuemao shen

doi:10.5582/ddt.2019.01053

Brief Report

Anti-virulence activities of biflavonoids from Mesua ferrea L. flower

Xiaochun Zhang, Rongrong Gao, Yan Liu, Yuhe Cong, Dongdong Zhang, Yu Zhang, Xuefei Yang, Chunhua Lu, Yuemao shen

Author information

Xiaochun Zhang
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University
Rongrong Gao
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University
Yan Liu
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University
Yuhe Cong
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University
Dongdong Zhang
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences
Yu Zhang
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences
Xuefei Yang
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences
Chunhua Lu
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University
Yuemao shen
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University

Keywords: Mesua ferrea (Roxb.) L., anti-virulence activity, biflavonoids, T3SS

JOURNAL FREE ACCESS

2019 Volume 13 Issue 4 Pages 222-227

DOI https://doi.org/10.5582/ddt.2019.01053

Details

Abstract

Based on the anti-virulence activity on Salmonella, the ethyl acetate extract (EAE) of Mesua ferrea flower was investigated for its chemical constituents. Ten purified compounds were identified and assayed for their inhibitory activity against Type III secretion system (T3SS) by polyacrylamide gel electrophoresis (SDS-PAGE) and Western blots experiments. We found the biflavonoids, rhusflavanone and mesuaferrone B, exhibited inhibitory effects on the secretion of Salmonella pathogenicity island 1 (SPI-1) effector proteins (SipA, B, C and D) without effecting the bacterial growth. In addition, 5, 6, 6'-trihydroxy-[1,1'-biphenyl]-3,3'-dicarboxylic acid (6) is a new natural product from M. ferrea flower.

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