Association of Advanced Glycation End-products (AGEs) in Blood with Amount of Body Fat, Glucose and Lipid Metabolism in Obese Young Subjects

Abstract

The present study was designed to investigate the association of serum advanced glycation end-products（AGEs）with body fat, glucose and lipid metabolism in obese young subjects and to examine the effects of instruction in improvement in diet and physical activity on the formation and accumulation of serum AGEs in 6 months. Forty-six young obese students（21±3.0yr, body weight: 83.2±18.1kg, BMI: 28.9±5.4kg/m2, body fat: 23.5±10.4kg）were recruited into the present study. Serum total cholesterol, HDL-cholesterol, triglyceride, fasting glucose, insulin, adiponectin, leptin and reactive oxygen metabolite（ROMs）levels were determined in all participants. Insulin resistance was assessed using Homeostasis Model Assessment（HOMA）-index. AGEs（3-DG, Pentosidine, and CML）were also evaluated in plasma by immunoassay. Six months after instruction, the measurement was repeated. Positive correlations of serum CML levels with HOMA-index（p=0.029）and with reactive oxygen metabolite levels（p=0.005）were observed. Six months after instruction, body weight, BMI, body fat, fasting glucose, plasma ROMs were significantly reduced in subjects who could be followed up（n=8）. Especially, positive correlations of decrease in CML levels with decrease in %body fat（p=0.007）were observed. These data indicate the presence of formation of circulating AGEs associated with latent insulin resistance and systemic oxidative stress in young obese adolescents without evident diabetes. And, in an early stage of life in obese subjects, life style modification（healthy diet and exercise training）might result in reduction in circulating AGEs formation accompanied with improvement in body composition and insulin resistance, possibly leading to prevention of the development of arteriosclerosis.