The Influence of Nitric Oxide-induced Vasodilatation on Blood Pressure During Hypoxic Adaptation - Risk Reduction of Erythrocytosis in Hypoxic Training Model Mice -

Abstract

Altitude training has been widely adopted by athletes, however it also involves a risk of excessive hematopoiesis. We used the model mice of hypoxic training (prolyl hydroxylase domain 2 conditional knockout mice: Phd2 cKO) to investigate the effect of erythrocytosis on blood flow and blood pressure. Additionally, we determined the effect of NOS inhibitor to the maintenance of blood pressure on Phd2 cKO mice. Phd2 cKO mice exhibited erythrocytosis and the reduction of blood flow velocity in hind limbs. As for systolic blood pressure, there was no significant difference between Phd2 cKO mice and control mice without treatment. 5 minutes after the injection of low concentration of NOS inhibitor, control mice exhibited an elevated systolic blood pressure. On the other hand, systolic blood pressure of Phd2 cKO mice was not affected by low concentration of NOS inhibitor. These results suggest that Phd2 cKO mice may be less susceptible to NOS inhibition. To understand the effect of NOS inhibition on blood pressure regulation in the state of polycytosis in more detail, further studies about concentration-dependent effect of NOS inhibitor using Phd2 cKO mice are required.