Study on the Quantification of Macroautoradiography

Abstract

The relationship between photographic density with radioactivity of particle emitters and exposure period was studied for quantification of macroautoradiography.
Photographic density was recorded over 3 different types of X-ray high sensitive films with time by contacting with 7 different radioactivity sheets of autoradiographic standard kits, ¹⁴C-polymethacrylate.
A good correlation was obtained between radioactivity and photographic density for during a 2 or 3 day exposure period. There was also a good relationship between exposure period and photographic density in case of low radioactivity of 0.025-0.75 μCi/g in the sheets. This relationship was disturbed by unequivalent combination of the radioactivity and exposure period.
Discussion was presented to clarify the analysis of such a complex relations as mentioned above.