Abstract
The effects of culture conditions on the formation and growth of adventitious bud derived from hairy roots of Atropa belladonna L. were investigated. Various concentrations of kinetin (KN) alone were prepared in combination with Murashige and Skoog (MS) and Gamborg's B5 (B5) media. Although the number and weight of adventitious buds were increased in MS medium, no marked effects by KN were shown. In the effects of sucrose concentrations, the number and weight of adventitious buds were most increased at 1% sucrose in MS medium. In the effects of inorganic components in MS medium, the strengths of inorganic solutions were modified, but the results were almost the same as a whole. The strong effects of ammonium nitrogen and thiamine were assumed in comparison with B5 medium. For inhibition of clump formation by shear conditions, independent adventitious buds were observed from 100 rpm rotary speed. Under the highest speed at 140 rpm, whereas the weight of adventitious buds was decreased, we found that the number was most increased as a positive effect of shear stress. Furthermore, multiple shoots with roots were regenerated from minute adventitious buds, so the possibility of mass seedling production from hairy roots of A. belladonna was suggested.
