Effect of Aluminum Chloride on the Organogenesis of Two Types of Cymbidium In Vitro

Abstract

We examined the effects of aluminum chloride (AlCl₃) on the organogenesis of Cymbidium Sweet Waffle ‘Tarte’ from inoculated protocorm-like bodies (PLBs) and of Cymbidium kanran ‘Murotonishiki’from rhizomes to determine the appropriate concentration of AlCl₃ for organogenesis. The explants were cultured in modified MS media supplemented with various concentrations of AlCl₃ and maintained at 25±1℃ and a 24 h light period for 6 weeks for C. Sweet Waffle‘Tarte’and 10 weeks for C. kanran‘Murotonishiki.’ In C. Sweet Waffle ‘Tarte’ 1.0 mg L^-1 AlCl₃ significantly increased PLB formation, shoot formation from PLBs and root formation from shoot. In C. kanran ‘Murotonishiki,’ 1.0 mg L^-1 AlCl₃ induced shoot from rhizome and developed roots from shoot. On the other hand, high concentration of AlCl₃ (10 mg L^-1) resulted the formation of protocorm-like shoots in both Cymbidiums. We concluded that the concentration of 1.0 mg L^-1 AlCl₃ is optimum for the root and shoot formation of the Cymbidium orchids.