Abstract
In order to induce and select amino acid analogue resistant mutants, a protocorm-like body (PLB) was induced by a shoot apex culture in a banana (Musa acuminata, (AAA) ) . And the growth inhibition of the PLBs by proline analogue (L-hydroxyproline) was studied.
The meristem including adjacent corm tissue was removed from the cultured shoot apex and was transplanted to a fresh medium. PLBs were induced on the sliced corm. They were proliferated by separating into smaller pieces and subculturing on the same medium.
L-hydroxyproline (HYP) containing media were prepared by adding HYP into the culture medium, after filter-sterilization. The growth inhibition of PLBs by HYP was analyzed by culturing PLBs on the HYP-medium. It was observed that concentrations of HYP up to 4 mM completely inhibited the PLB's growth, i.e. prevented any increase of the fresh weight of the PLBs.
The results described here are being used for the selection of banana tolerant to the amino acid analogue.
