Abstract
Effect of types of culture vessels with supporting materials on the growth of carnation plantlets in vitro has been investigated. The experiment was carried out using Sorbarod System (plastic vessel) with Sorbarod (fibrous cellulose plug) ; called SS hereafter, Star Pac (gas permeable plastic bag) with agar; called SP, test tubes with agar capped with gas permeable plastic film; called TF and test tubes with agar capped with aluminum foil; called TA. The numbers of air changes per hour of SS, SP, TF, and TA vessels were, respectively, 9.6, 0.23, 5.3, and 0.32. The experiment was carried out at a photosynthetic photon flux of 60 μmol⋅m-2 ⋅sec-1 (ca. 4.5 klx) for 16 hr per day and a room air temperature of 25°C.
On day 40, the greatest average fresh weight of plantlets, the largest percent dry matter, the smallest top/root fresh weight ratio and the shortest shoot were observed in Treatment SS. The percent dry matter, top/root fresh weight ratio and shoot length in Treatment TF were similar to those in Treatment SS. Some multiple shoots were observed in Treatment SP. Some plantlets were vitrified in Treatments SP and TA.
The results indicate that the combination of the culture vessels with a high number of air changes per hour and the fibrous supporting materials promotes the growth of carnation plantlets in vitro.
