Abstract
To clear effects of injury and plant hormone treatments to mother-scale on the bulblet formation of Lilium japonicum Thunb. the experiments were conducted. The scales cut off a part of abaxia and the edge of the mother-scale were used as explant. And they were cultured on MS medium without plant hormones at 24°C for 8 weeks under continuous light of 20 μmol⋅m-2⋅s-1. The number of bulblets formed on the explant was about twice of control and there is a significant difference between the injury-treatment and control. The mother-scale was cut into 8 pieces of explant from basal to apical portions. The explants were planted on MS medium containing 2, 4-D 1.0 mg/L and kinetin 0.1 mg/L, 2, 4-D 0.1 mg/L and kinetin 1.0 mg/L, or without hormones. And they were cultured for 8 weeks under the same conditions as the experiment 1. The combination of 2, 4-D 1.0 mg/L and kinetin 0.1 mg/L exhibited the most effect on the bulblet formation. Size of bulblets on the basal part of the scale was the largest in all explants and the number of bulblets formed on the middle part was more than ones in the basal and apical parts of the scale.
