Abstract
As an inoculum source the mycelium was isolated from fruiting-body of Tricholorna giganteum which collected in the Ogasawara. The used culture medium was potato-dextrose yeast agar (PDYA) medium containing LVD (prepared from softwood sulphite pulp waste) . The optimum conditions of mycelial growth were initial pH 5.5 and D-mannose as carbon source, and the best promotional concentration of LVD added to medium was 0.8%. In the artificial cultivation the medium containing hardwood sawdust, rice bran and wheat bran (400: 23: 35v/v) and keeping 60-65% moisture was employed. In the bottle culture polypropylene bottle containing the above medium (550g) was used and cultured at 24°C. After 40 days culture, surface of the medium in bottle were covered with rice hulls, sand or farm soil and the bottles were cultured at 26-27°C and 85-90% humidity to form the fruiting-body. In these cultivations, farm soil gave the best formation of fruiting-body. The yeilds of fruiting-body on the floor and shelving in the green house were 670 and 600 g per a bag containing 2.5 kg medium, respectively.
