Abstract
Bulblets regenerated on the scale of Lilium japonicum Thunb. are induced into dormancy during in vitro culture. We conducted the experiment to know the effects of cold temperature, 24-epibrassinolide (EB) and light on breaking of the dormancy of the Bulblets. 1. The rate of leaf emergence from the small bulblets (35 to 50 mg, FW), pre-treated at 4°C for 8 weeks and cultured on MS-medium without plant hormones under light, was 85%. 2. The rate of leaf emergence from the large bulblets (1 to 2 g, FW), without cold treatment and cultured on MS-medium containing 0.1 ppm EB, was 54%. However the bulblets, which were pre-treated at 4°C for 10 weeks and cultured on media containing 0.1 and 1 ppm EB under light, showed 100% of leaf emergence. 3. After the bulblets were cultured on the media under the light or the dark in vitro and treated at 4°C for 10 weeks, they were transplanted into pots. The rate of leaf emergence from the bulblets grown under the light was higher than the dark. But dormancy of the bulblets both under the light and the dark wasn't completely broken out. After the bulblets were harvested from the pot and re treated at 4°C for 10 weeks, they were retransplanted into a new pot. The bulblets both under the light and the dark indicated 100% of leaf emergence.
