In Vitro Propagation of Toad Lily (Tricyrtis hirta Hook.) by Repeated Stem-sectioning and Division of Axillary Shoots

Abstract

Micropropagation of toad lily (Trichyrtis hirta Hook.) was achieved by subculturing stem-sections with nodes and divided axillary shoots on Murashige and Skoog (MS) basal medium with 0, 0.44 or 4.4μM N⁶-benzyladenine (BA) . A proliferation rate of 3.1 microcuttings per 17-d interval was achieved on the medium with 4.4μM BA. Shoots elongated from these in vitro cultured explants could be rooted at the frequency of 90% on the MS medium with 0.49μM indole-3-butyric acid (IBA) . Plants were readily acclimatized in the soil and flowered normally after transfer to a greenhouse.