Abstract
The effects of cytokinins added to MS media on the growth of apical and nodal stem segments of axillary branches of Gloriosa superba L. were examined. Survival rates of explants were increased by the addition of BA and TDZ. The optimal responses were obtained by addition of TDZ at concentrations higher than 0.3μM. Thidiazuron was effective on shoot and tuber formation in both apical and stem segment cultures. The optimal shoot proliferation was obtained at 3 and 10μM TDZ treatments (average number: 1.6/segment) . Higher number and rate of tuber formation were obtained in stem segments with TDZ at concentrations higher than 3 μM. The tuber formation markedly increased on MS medium at 10μM TDZ (average number: 7.8/segment, formation rate: 96%) .
