Abstract
An efficient micropropagation system using the root culture were established in Oenanthe javanica DC. In the shoot tip culture, the tip portion of terminal bud or lateral bud was excised and cultured on the half strength Murashige and Skoog medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA) or combination of 0.2 mg/L NAA and 0.2 mg/L 6-benzyladenine (BA) under continuous light of 45 μmol m-2s-1 at 25°C. In root segment culture, root explants which were whole roots excised approximately 1.5 cm length from aseptically generated plantlets were used and cultured on MS medium supplemented with 0.05, 0.1 or 1 mg/L 2, 4-dichlorophenoxy acetic acid (2, 4-D) and 0, 0.01 or 0.1 mg/L BA at 25°C in the dark. Embryogenic calli were induced on medium supplemented with 0.1 mg/L 2, 4-D and 0, 0.01 or 0.1 mg/L BA. Plant regeneration was successful on the MS hormone-free medium. Many embryos were developed into normal plants by subculture on the same medium. In vitro propagation with root and petiole sections of aseptically generated plants in vitro is more efficient than conventional shoot division ex vitro. The results indicate that the micropropagation procedure can be applied to an efficient propagation of Oenanthe javanica DC.
