2016 Volume 74 Issue 3 Pages 51-59
Objective: In the non-obese condition, the effect of low carbohydrate-high protein (LC-HP) diets on oxidative stress leading to atherosclerotic disease is unclear. We investigated the impact of an LC-HP diet on oxidative stress for a short (2 weeks) and long (13 weeks) period, using non-obese mice.
Methods: Six-week-old C57BL/6J male mice were given either the LC-HP (PFC energy ratio: 40, 16, 44) or control (PFC energy ratio: 20, 16, 64) diet for either 2 or 13 weeks.
Results: In the LC-HP-diet group, the bodyweight gain and epididymal adipose tissue weight and adipocyte area were significantly lower and kidney weight was higher than those in the control. Hepatic superoxide dismutase (SOD) activity was significantly lower in the LC-HP-diet group than in the control. The LC-HP diet did not affect serum levels of adipocytokines, insulin, or hepatic TBARS—an indicator of oxidation products, or lectin-like oxidized LDL receptor-1 ligand containing ApoB—an indicator of oxidized LDL.
Conclusions: Our results suggest that an LC-HP diet in non-obese mice inhibited bodyweight gain and epididymal adipocyte area. Additionally, the LC-HP diet might affect the development of arteriosclerotic diseases by increasing oxidative stress through the reduction of hepatic SOD activity.
