e-Journal of Surface Science and Nanotechnology
Online ISSN : 1348-0391
Conference -ISSS-7-
Surface Modification of Cell Scaffold in Aqueous Solution Using TiO2 Photocatalysis and Linker Protein L2 for Patterning Primary Neurons
Kohei SekineHideaki YamamotoSho KonoTakeshi IkedaAkio KurodaTakashi Tanii
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Volume 13 (2015) Pages 213-218

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Abstract

Titanium dioxide (TiO2) photocatalysis can be applied to pattern proteins and cells under aqueous solution. In this work, we extended the application of this technique to patterning primary neurons, a type of cell with relatively weak adhesibility. For this purpose, we employed ribosomal protein L2 (RPL2) that has high affinity toward silica and metal oxides, including TiO2, to stably bind a neuronal adhesion protein laminin to the TiO2 surface. We utilized two types of molecular recognition to achieve this—binding of anti-laminin antibody to its antigen (laminin) and binding of protein A to the antibody. We show that a protein complex consisting of laminin/anti-laminin antibody/protein A-RPL2 is spontaneously formed by simply mixing the precursor proteins in solution phase. We then show that the surface coated with the protein complex supports stable growth of rat hippocampal neurons. Finally, we show that the cells can be selectively grown on the protein complex patterned with the TiO2-assisted method. The protocol established in this work is a unique combination of a top-down micropatterning of the surface using TiO2 photocatalysis and a bottom-up self-assembly of biomolecules, which can be further applied to pattern a wide range of proteins and cells. [DOI: 10.1380/ejssnt.2015.213]

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