Detection of l-lactic Acid using Electrochemically-Controllable Chemiluminescence System

Abstract

11-Ferrocenyl-1-undecanethiol (FcC₁₁SH) self-assembled monolayer on gold electrode was used for the electrochemically-controllable catalyst for chemiluminescence of luminol. Ferrocene head groups adsorbed on gold were oxidized to ferricinium cation species electrochemically and then catalyzed the chemiluminescence of luminol. The light emission could be detected even in the neutral and in the weak acid solutions. The presented system was adopted for detection of l-lactic acid, which was well known as an important metabolite for clinical, food and bioprocess analysis, in the presence of lactate oxidase.