2006 Volume 74 Issue 8 Pages 642-644
Adsorption behavior of bilirubin oxidase (BOD) on Au electrodes was studied using quartz crystal microbalance (QCM) measurements and electrochemical measurements of BOD-catalyzed reduction current of O2 without mediators. The QCM measurements supported the monolayer adsorption of BOD on Au electrodes at pH 5 to 9. The catalytic current density was, however, much smaller than that at hydrophilic edge plane-type carbon electrodes or than the calculated value for active BOD. Some denaturation is suggested on Au electrodes, which was supported by electrochemical QCM (EQCM) measurements.