Fabrication of Genetic Diagnostic Chip using DNA-arrayed TFT Photosensor

Abstract

This work describes a disposable DNA-arrayed thin film transistor (TFT) photosensor integrating a miniaturized reaction unit and a photoelectronic effect-based internal detection sensor. Single stranded DNA was chemically conjugated and arrayed on the TFT photosensor, which was fabricated by the semiconductor integrated circuit (IC) technology, for the detection of specific target DNA by DNA-DNA hybridization. The photosensor surface can be modified by various silane coupling reagents for the optimization of oligonucleotide immobilization conditions and single nucleotide polymorphism (SNP) detection was successfully employed using the TFT photosensor. A microfluidic channels were constructed on the TFT photosensor to improve the hybridization efficiency. The developed TFT photosensor will further promote the development of disposable photodetecting devices applicable in genetic diagnosis.