ATP-mediated Release of a DNA-binding Protein from a Silicon Nanoneedle Array

Daisuke MATSUMOTO; Maui NISHIO; Yoshio KATO; Wataru YOSHIDA; Koichi ABE; Kyoko FUKAZAWA; Kazuhiko ISHIHARA; Futoshi IWATA; Kazunori IKEBUKURO; Chikashi NAKAMURA

doi:10.5796/electrochemistry.84.305

Communications

ATP-mediated Release of a DNA-binding Protein from a Silicon Nanoneedle Array

Daisuke MATSUMOTO, Maui NISHIO, Yoshio KATO, Wataru YOSHIDA, Koichi ABE, Kyoko FUKAZAWA, Kazuhiko ISHIHARA, Futoshi IWATA, Kazunori IKEBUKURO, Chikashi NAKAMURA

Author information

Daisuke MATSUMOTO
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Maui NISHIO
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
Yoshio KATO
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Wataru YOSHIDA
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
Koichi ABE
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
Kyoko FUKAZAWA
Department of Materials Engineering, The University of Tokyo
Kazuhiko ISHIHARA
Department of Materials Engineering, The University of Tokyo
Futoshi IWATA
Department of Mechanical Engineering, Shizuoka University
Kazunori IKEBUKURO
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
Chikashi NAKAMURA
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Keywords: Controlled Release, ATP Aptamer, Nanoneedle Array

JOURNAL FREE ACCESS

2016 Volume 84 Issue 5 Pages 305-307

DOI https://doi.org/10.5796/electrochemistry.84.305

Details

Abstract

We describe a new method for delivering a zinc finger protein directly into cells using a nanoneedle array and an ATP aptamer. The zinc finger protein can be used for site-specific DNA modification by combining with an enzyme that can cut or modify DNA. This controlled release method utilizes aptamer conformational changes to deliver various biomolecules specifically into cells.

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