Determination of Lactate by Sequential Injection Analysis Using a Fluoride Ion-selective Electrode Detector

Yuichi TAKAHASHI; Takashi MASADOME

doi:10.5796/electrochemistry.20-00097

Abstract

A combination of sequential Injection Analysis (SIA) system and the ion-selective electrode (ISE) detector is considered to be one of a very effective analytical method. Reaction of lactate with lactate oxidase produces pyruvic acid and hydrogen peroxide. When the produced hydrogen peroxide is reacted with 4-aminoantipyrine (4-AAP) and 4-fluorophenol (4-FP) in the presence of horseradish peroxidase (HRP), F⁻ ion is produced. The concentration of lactate can be indirectly determined by measuring the F⁻ ion by F⁻ ISE. Based on this principle of detection of lactate, a new measurement method of lactate using an SIA with ISE detector was developed.

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This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License (CC BY, http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse of the work in any medium provided the original work is properly cited. [DOI: 10.5796/electrochemistry.20-00097].
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