Continuing the data independent acquisition revolution: ZT Scan DIA for quantitative proteomics

Abstract

Data independent acquisition (DIA) has been a central catalyst for shifting mass spectrometry (MS)-based omics from a discovery-focused science to one that more comprehensively facilitates longitudinal studies, as required in clinical research and precision medicine. The challenges associated with improving throughput in high-resolution MS while maximizing depth, reproducibility, and quantitative precision, have given rise to ZT Scan DIA. The development of Zeno trap, a novel accumulation and pulsing device, enabled a new era of sensitivity for accurate mass instruments. In Zeno SWATH DIA, the Zeno trap, when activated, is used to increase the MS/MS sensitivity for each variable window acquired. The Zeno trap provides a 4-20x gain in sensitivity for Zeno SWATH DIA, while also maintaining other key performance attributes. In this technical overview we introduce a novel DIA approach, ZT Scan DIA, which combines the depth of DIA methods with the precision of targeted approaches. Like Zeno SWATH DIA, ZT Scan DIA enables the identification and quantitation of analytes using MS/MS data. Thus, the rapid acquisition of high-quality MS/MS data across the entire precursor ion space is fundamental to operation. With the added dimension of data from quadrupole scanning, ZT Scan DIA allows researchers to unravel the complexity of biological samples by extending depth and certainty in the quantitative measurement.