Studies on the Anti-gonadotrophin in the Blood of Patients Treated with Pregnant Mares' Serum Gonadotrophin

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From our experience in the treatment of anovulatory cycle with gonadotrophin (PMS and HGG) preparation it was observed that rather larger amounts of PMS were needed to succeed in the induction of ovulation in the repeated series of treatment than in tne first series (Table 1). This evidence is presumed to be due to the appearance of anti-PMS in blood.
Blood specimens were drawn from nine patients selected from the group who were treated with gonadotrophin. Anti-PMS titres were examined by using the haemagglutination reaction immunologically before and after the treatment (Table 2). Although no anti-PMS titres were observed in all the cases before the treatment, they markedly increased after treatment with some individual differences. Blood specimens from seven patients who were treated with repeated gonadotrophic therapy 15-30 weeks after the first therapy, were tested by the same method (Table 3). In some of them anti-PMS titres were still seen before the second series of injections. Generally, titres of anti-PMS increased rapidly and continued at high level after the second treatment.
Biologically, 1 ml of serum obtained from a patient who showed 1,024 of anti-PMS titre inactivated completely 2 IU of PMS in uterine weight on CF 1 female immature mouse (Table 4). This anti-serum was diluted by the 2-fold method and tested in the same way. As a result it was recognized that 1 ml of anti-serum up to 64 of titre did not neutralize 2 IU of PMS (Table 5). Specimens drawn from six patients who showed various different titres gave the same results as above (Table 6).
These results obtained indicate that anti-PMS which can neutralize PMS activity appears after the successive injection of PMS in all cases. For this reason gonadotrophic therapy must be carried out carefully with a rational schedule.