Abstract
Recently the following decapeptide sequence for porcine luteinizing hormone releasing factor (LRF) was proposed by Schally's group : (Pyro) Glu-His-Trp-Ser-Tyr-Gly-Leu Arg-Pro-Gly-NH2. According to this report, many investigators have succeeded in synthetizing LRF and this synthetic LRF is now available for clinical study. Then, synthetic LRF (MO-1208 and DB-2521) was tested in the human being. Two hundred micrograms of synthetic LRF was injected into the following 21 women and serum LH and FSH response was tested by double antibody radioimmunoassay : 4 volunteers of follicular phase, 4 volunteers of luteal phase, 5 anovulatory females of amenorrhea with estrogenic activity and 8 anovulatory females of amenorrhea without estrogenic activity. Single iv injection of 200 micrograms of synthetic LRF induced a significant increase in LH release into the blood within 30 minutes and an increase in FSH release within 45 minutes in 20 of 21 subjects. Only one exception was a case of hypopituitarism and no response of LH and FSH release was to be found after injection. This study demonstrate that synthetic LRF will be utilized effectively for the study on the function of hypothalamopituitary gonadal axis.
