Abstract
In our previous report (S. Aonuma et al. 1973), it was demonstrated that the guinea pig spermatozoa released the sperm specific substance in estrogen-dominated uterus much greater than that in the progesterone-dominated uterus. This released substance had the ability to block the fertilization of rabbit capacitation spermatozoa (decapacitation). From the viewpoint that the release of decapacitation factor (DF) from the spermatozoa stimulates the sperm capacitation process, it is suggested that the spermatozoa capacitation is induced in the suitable chemical defined medium in which DF is released from the spermatozoa without damages to the spermatozoa.
This present data demonstrated the induction of rabbit and guinea pig sperm capacitation in vitro.
