1987 年 63 巻 2 号 p. 112-122
An enzyme-linked immunosorbent assay (ELISA) for 17α-hydroxyprogesterone (17- OHP) in dried blood collected on filter paper has been developed. The ELISA method is very easy and rapid and has sensitivity, accuracy and precision. The detection limit of the ELISA method is 0.5 ng/ml blood. Intra-and interassay coefficients of variation are 7.0 “- 8.3% and 6.7”-10.5%, respectively. Correlation between the ELISA method and previously reported enzyme immunoassay is good (r = 0.961).
17-OHP values of neonates with congenital adrenal hyperplasia (CAH) are extremely high compared with normal neonates (11.0 ± 5.9 ng/ml blood).
The present study demonstrates that the ELISA method for 17-OHP is applicable to neonatal screening for CAH.