Abstract
3H-leucine uptake of rat adenohypophysial cells was studied autoradiographically in vitro. The autoradiographs were stained with PAS, metanil yellow and toluidine blue. In organ-cultured pituitaries PAS-positive cells showed a significantly lower uptake rate than acidophils and chromophobes on the 2nd and 4th day of cultivation. The uptake of these three type cells was increased with the time of exposure to 3H-leucine. However, the increase in the former was much less than that in the latter two.
The influence of acidic hypothalamic extracts was studied using short term incubation method. When hypothalamic extracts were added to incubation media, the uptake of 3H-leucine by PAS-positive cells rose to a considerable higher level than that found in the other two types. The uptake rates of acidophils and chromophobes were unchanged by the addition of hypothalamic extracts.
