1965 Volume 12 Issue 2 Pages 78-82
Crude and purified β-parotin obtained from fresh parotid gland were biologically inactive, while the same products obtained after the autolysis of the gland were active. However, it was found that the inactive material could be easily activated by the incubation at 37°C; the most suitable condition was 24 to 48hrs. incubation at pH 5.5 in an aqueous solution.
Although the mode of activation is still remained to be clarified, it seems to occur without an activator, because the activation was observed even with a highly purified material.