BIOCHEMICAL STUDIES ON HUMAN SALIVARY PROTEINS I FRAGMENTS WITH HYPOCALCEMIC ACTIVITY IN ENZYMATIC DIGEST OF SALIVA-PAROTIN-A

Abstract

The degraded products of saliva-parotin-A (SPA) by Pronase P were investigated chemically and biologically. They were separated into 6 fractions by paper chromatography and the Rf value, recovery, chemical properties, and solubility of each fraction were determined. The maximum ultraviolet absorption was found at a wave length of 271mμ for Fraction 1, and 279mμ for SPA. The molecular weight of Fraction 1, which was the most active fragment, was estimated to be 400-500 by gel filtration through Sephadex G-25.